Alg-SAC0: | Alginate modified with succinic anhydride |
Alg-SAC12: | Alginate modified with dodecenyl succinic anhydride |
AOs: | Alginate oligosaccharides |
BCA: | Bicinchoninic acid (protein assay) |
BLG: | |
CAL-B: | |
CRL: | |
DP: | Degree of polymerization |
PC: | Phosphatidyl-choline |
ROS: | Reactive oxygen species |
TBARS: | Thiobarbituric acid reactive species |
Reagents, solvents, and solutions | |
ABTS: | 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) |
DMAP: | 4-(N,N-dimethylamino)pyridine |
EDTA: | Ethylenediaminetetraacetate dehydrate |
PBS: | Phosphate buffered saline |
SAC0: | Succinic anhydride |
SAC12: | Dodecenyl succinic anhydride |
TBA: | Thiobarbituric acid |
TRIS: | Tris(hydroxymethyl)aminomethane |
Techniques: | |
CLSM: | Confocal laser scanning microscopy |
ESI-MS: | Electrospray ionization mass spectrometry |
ELSD: | Evaporative light scattering detection |
FTIR: | Fourier transformed infrared spectrometry |
HPLC: | High performance liquid chromatography |
PDA: | Photodiode array detection |
SEC: | Size exclusion chromatography |
TLC: | Thin layer chromatography |
Starting material | Alginate lyase | Buffer / pH / temperature | DP | Reference |
---|---|---|---|---|
Sodium alginate from |
Alginate lyase from |
50 mM TRIS-HCl / pH 7.5 / 28°C | 2, 3, 4 | (Zhang et al. 2004) |
Sodium alginate (commercial, unspecified) | Recombinant exo-alginate lyase Alg17C from |
20 mM TRIS-HCl / pH 6.0 / 40°C | 1 | (Kim, Chung, et al. 2012) |
Sodium alginate (commercial, unspecified) | Recombinant endoalginate lyase Alg7D from |
20 mM TRIS-HCl / pH 7.0 / 50°C | 2, 3, 4, 5 | (Kim, Ko, et al. 2012) |
Sodium alginate from brown algae (commercial) | Alginate lyase aly-SJ02, from |
50 mM TRIS-HCl 0.25 mM NaCl / pH 8.5 / 50°C | 2, 3 | (Li et al. 2011) |
Homopolymeric guluronic acid | Alginate lyase (unspecified) | 50 mM TRIS-HCl / pH 7.5 / 30°C | 2, 3 | (Liu et al. 2002) |
Homopolymeric polymannuronate | Haliotis tuberculata alginate lyase | 50 mM TRIS-HCl / pH 7.5 / 25°C | 2, 3, 4, 5 | (Heyraud et al. 1996) |
Homopolymeric polyguluronate | Klebsiella pneumoniae alginate lyase | 50 mM TRIS-HCl / pH 7.5 / 20°C | 2, 3, 4 | (Heyraud et al. 1996) |
Reaction | Glucuronic acid [mmol] | Oleic acid [mmol] |
---|---|---|
1 | 1.0 | 1.0 |
2 | 2.0 | 1.0 |
3 | 5.0 | 1.0 |
4 | 1.0 | 5.0 |
5* no lipase | 1.0 | 1.0 |
6 | 5.0 | 1.0* lauric acid |
Reaction | Adjuvant |
---|---|
7 | None |
8 | 5 g acetone |
9 | 5 g 2-butanone |
Reaction | Molecular sieves (100 mg) | Silica gel (136 mg) |
---|---|---|
10 | + | + |
11 | + | - |
12 | - | + |
13 | - | - |
Reaction | TLC | ESI-MS |
---|---|---|
1 | + | NA |
2 | + | NA |
3 | ++ | + |
4 | - | NA |
5 | - | NA |
6 | ++ | + |
7 | - | NA |
8 | - | NA |
9 | - | NA |
10 | - | NA |
11 | - | NA |
12 | - | NA |
13 | - | NA |
14 | NA | - |
Stabilizer | Mean droplet size (d |
PDI | pH | Zeta potential (mV) |
---|---|---|---|---|
DSA | 7.64 ± 1.06 | 0.483 ± 0.239 | 6,7 | −32.1 ± 0.9 |
Alginate | 22.02 ± 10.16 | 0.784 ± 0.216 | 6,1 | −22.8 ± 0.8 |
BLG | 3.50 ± 1.18 | 1 ± 0 | 6,7 | −11.6 ± 1.1 |
|
||||||
|
||||||
|
||||||
|
||||||
|
||||||