Experiments in Cell and Molecular Biology
A Laboratory Manual for Undergraduate Instruction or Concepts and Techniques for the Undergraduate Laboratory
Laboratory Manuals for Life Sciences
Author: Miguel Cervantes-Cervantes Rutgers, The State University of New Jersey, Newark, NJ, USA
ISBN: 9788743813200 (Paperback)
Available: September 2026
Experiments in Cell and Molecular Biology invites students beyond observation and into discovery. Designed for advanced-freshman/sophomore-level and above, this laboratory manual offers ten carefully scaffolded experiments that build from foundational concepts to more advanced investigative techniques in modern cell and molecular biology.
Through hands-on experimentation and guided experimental design, students explore the chemical components of the cell, subcellular structure, metabolism, membrane biology, signal transduction, and the molecular basis of gene expression and regulation using eukaryotic model systems. Suitable as a companion to a lecture course or as a stand-alone laboratory experience, this manual emphasizes critical thinking, data interpretation, and hypothesis-driven science.
Ideal for students preparing for advanced coursework and careers in the life sciences, Experiments in Cell and Molecular Biology endows learners with the conceptual understanding and practical skills essential for studying the cell from a molecular perspective.
Description, Prerequisites, Mission Statement
Learning Goals
Learning Objectives
General Laboratory Instructions
Laboratory No. 1: Bioinformatics: Self-guided Internet-based Exercise on Databases for
the Storage and Data Mining
Laboratory No. 2: Biological Buffers I. Titration of the Amino Acid Glycine
Laboratory No. 3: Biological Buffers II. Preparation of a Phosphate Buffer
Laboratory No. 4: Determination of Protein Concentration using the Bradford Method
Laboratory No. 5: Enzyme Kinetics
Laboratory No. 6: Assay of Cytochrome c Oxidase in Potato Tuber Mitochondria
Laboratory No. 7: Molecular Evolution: Separation of Fish Proteins Using Polyacrylamide Gel Electrophoresis
Laboratory No. 8: Genetic Fingerprinting
Laboratory No. 9: Molecular Biology of Sickle-cell Anemia
Laboratory No. 10: Signal Transduction: Antagonistic effects of Gibberellin
and Abscisic Acid on the Production of a-Amylase in Barley Seeds